Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Index-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
relative quantification B-6
automatic outlier removal 6-19
Automation Accessory
components 7-36
Plate Handler, See Plate Handler
See fixed-position bar code reader
Automation Controller Software
about 4-39
adding plates to the plate queue 4-40
configuring for operation 4-42
ejecting a plate 4-44
launching 4-39
monitoring instrument progress 4-44
removing plates from the plate queue 4-40
starting the plate queue 4-43
stopping the plate queue 4-44
B
background run 7-16 to 7-19
about 7-16
constructing a background plate 7-17
creating a plate document 7-17
extracting 7-19
preparing a background plate 7-17
troubleshooting 8-9
when to perform 7-16
bar code information
entering into a plate document 2-27, 3-28
bar code readers
fixed position, See fixed-position bar code reader
hand held, See hand-held bar code reader
bars, in the Gene Expression Profile
symbols 6-31
baseline
about D-6
configuring value for automatic analysis 6-11
Block readout (from the Real Time tab) 4-27
Bucket Type, setting on centrifuge 4-13
C
calibrating the 7900HT instrument
adjusting the plate-sensor switch 7-37
aligning the fixed-position bar code reader 7-49
aligning the Plate Handler 7-41
performing background run 7-16
performing pure dye (spectral) run 7-20
calibrator sample 6-20
Centrifuge system
bucket requirements 4-16
Bucket Type, setting 4-13
components 4-12
installation and operation 4-13
placing bucket in centrifuge 4-17
requirements 4-12
TaqMan Low Density Array, placing in
bucket 4-16
Centrifuging, TaqMan Low Density Array 4-16
changing
gripper finger pads 7-52
pane, view, and plot sizes 2-21
sample block module 7-6
checklists
absolute quantification 6-9
allelic discrimination 5-10
dissociation curve 6-22, 6-40
relative quantification 6-22
chemistry
5´ Nuclease (TaqMan) D-2
non-optimized 8-6
SYBR Green D-3
troubleshooting 8-5 to 8-8
cleaning
gripper finger pads 7-52
sample block wells 7-15
closing
instrument tray (Automation Controller
Software) 4-44
instrument tray (SDS software) 4-29
plate documents 2-18
Zymark Twister Software 7-48
comments
adding to a detector 3-11
adding to a plate document 3-28
Comparative C
T
Method
about 6-4, D-8
formula derivation D-8
Components
centrifuge system 4-12
TaqMan Low Density Array 4-10
computer
about 1-6
hard drive partitions 1-6
maintaining 7-54
minimum system requirements 1-6
troubleshooting 8-14
turning ON 2-4
configuring the 7900HT instrument for 9600
emulation 3-21
consumables
384-Well Optical Reaction Plates C-3
96-Well Optical Reaction Plates C-4
improper or damaged plastics 8-8
Optical 96-Well Fast Thermal Cycling Plates C-4
Optical Adhesive Covers C-3
Optical Cap Strips C-3
writing on reaction plates 8-7
Contamination
genomic DNA 4-11
preventing 4-6
contamination
decontaminating the sample block 7-14

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English