Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Index-3
fluorescent, common sources 8-7
isolating on the sample block module 8-9
contextual menus, using 2-28
Control assay 4-11
copying
detectors to a plate document 3-13
markers to a plate document 3-15
Cover readout (from the Real Time tab) 4-27
creating
background plate documents 7-17
custom pure dye plate documents 7-28
detectors 3-11
markers 3-14
plate documents 2-13, 3-9
plate documents from a template 3-26, 4-36
pure dye plate documents 7-22
C
T
, See threshold cycle
custom pure dyes
adding to the pure dye set 7-27 to 7-29
creating a custom pure dye plate 7-27
cycle set, adding to a method 3-21
D
data type definitions (exportable) A-17
database
saving plate documents 4-24
saving results 5-19, 6-53
saving templates 3-25
database connection A-22
decontaminating the sample block module 7-14 to 7-15
defragmenting the hard drive 7-54
deleting, steps from a method 3-21
detector tasks
about 3-16
applying 3-16
importing into a plate document A-2
detectors
about 3-11
applying to a plate document 3-13
copying to a plate document 3-13
creating 3-11
importing into a plate document A-2
tasks, See detector tasks
determining melting temperatures 6-44
diagram
database LAN setup 1-23
database WAN setup 1-24
disconnecting the SDS software 4-30
display settings, adjusting 3-24
dissociation curve
about 6-38
analyzing 6-40 to 6-44
definitions of the T
m
value 6-43
procedure checklist 6-22, 6-40
programming a temperature ramp 3-23
Dissociation Plot
about 6-42
exporting as a graphic file A-16
exporting data as a text file A-16
E
ejecting a plate from the 7900HT instrument 4-29,
4-44
endogenous control 6-20
end-point runs
about 5-2
allelic discrimination, See allelic discrimination
entering
bar code information 2-27, 3-28, 4-36
comments 3-11, 3-28
sample names into a plate document 3-28
Equipment needed 4-12
exportable data type definitions A-17
exporting
data from a plate document 2-14, A-16
plots and views as graphic files A-16
External endogenous control assay 4-11
F
finger pads
cleaning 7-52
replacing 7-52
fixed-position bar code reader
aligning 7-49 to 7-51
connections 1-10
location 1-7
specification 1-7
fluorescent
contamination 8-7
detection system 1-10
fluorogenic probe
about D-2
designing B-2 to B-3
G
gene expression level
maximum 6-33
minimum 6-33
gene expression levels 6-31
Gene Expression Plot 6-31
Genomic DNA contamination, minimizing 4-11
genotypic segregation of datapoints
(Allelic Discrimination Plot) 5-8
Geometric (Exponential) Phase D-4
graph, Gene Expression Profile
sample bars 6-31
X-axis 6-31

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English