Applied Biosystems 7900HT - Page 378

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Index-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Y-axis 6-31
grey dividing line, using 2-21
grid, See plate grid
gripper 7-36
guidelines
absolute quantification assay development B-6
allelic discrimination assay development B-5
loading the Plate Handler 4-41
relative quantification assay development B-6
TaqMan probe design B-2 to B-3
H
hand-held bar code reader
connections 1-10
location 1-7
specification 1-7
using 2-27
hard drive
defragmenting 7-54
partitions 1-6
heated clamp 1-4
help
background information 2-3
using the Sequence Detection Systems Software
Online Help 2-3
hold, adding to a method 3-21
hotkey combinations, using 2-28
I
importing setup table data 2-16, A-2
imprecise pipetting 8-6
improper threshold setting 8-5
installing
plate adapter 7-12
sample block module 7-6
SDS software 7-55
the operating system software 7-55
instrument 1-4
about 1-2 to 1-10
external components 1-3
firmware 1-14
internal components 1-4
loading plates 4-25
maintaining 7-4
optics system 1-5
status lights 2-5
supported runs and chemistries 1-2
troubleshooting 8-14
turning ON 2-4
instrument tray 4-44
opening and closing (Automation Controller
Software) 4-44
opening and closing (SDS software) 4-29
replacing the plate adapter 7-12
IP Address A-22
irreproducibility
causes 8-5 to 8-8
J
Java Runtime Environment, about 1-14
K
keyboard shortcuts, using 2-28
L
launching
Automation Controller Software 4-39
SDS software 2-7
LAVA software
about 1-14
aligning the fixed-position bar code reader 7-49 to
7-51
launching 7-49
LDHost software, See LAVA software
learning to use the SDS software 2-10
lights, See instrument, status lights
limited warranty statement E-1
Linear Phase of the amplification curve D-5
loading plates
into the instrument 4-25
into the Plate Handler stacks 4-42
low copy templates 8-8
M
maintenance schedule 7-4
managing SDS data 1-17
markers
about 3-14
applying to a plate document 3-15
copying to a plate document 3-15
creating 3-14
importing into a plate document A-2
master mixes
preparing B-3
using 8-6
maximizing, instrument throughput 3-4
maximizing/minimizing panes, views, and plots 2-22
maximum
gene expression level 6-33
melting temperature
definition 6-43
determining 6-44
methods
about 3-19 to 3-23
adding a hold, cycle set, or step 3-21
adding a temperature ramp 3-23