Applied Biosystems 7900HT - Page 379

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Index-5
adjusting step parameters 3-21
configuring data collection options 3-22
programming 3-20 to 3-23
removing a step 3-21
setting the sample volume 3-22
minimum
gene expression level 6-33
modes of operation 1-17
automated 1-18
database 1-18
stand-alone 1-17
mRNA targets, number per well 4-10
N
no template control (NTC)
detector task 3-16
NTC calls applying 5-15
NTC calls verifying 5-16
Number of targets per well 4-10
O
↓ 6-33
opening
instrument tray (Automation Controller
Software) 4-44
instrument tray (SDS software) 4-29
plate documents 2-19
operating system
supported 1-6
upgrading 7-55
operating the 7900HT instrument
power switch 2-4
optimizing primer and probe concentrations B-3
outliers
allelic discrimination 5-8
automatic outlier removal 6-19
outlying amplification
multi-reporter experiments 6-19
P
panes
hiding 2-21
maximizing/minimizing 2-22
resizing 2-21, 2-22
showing 2-21
passive reference
setting 3-18
PCR
5´ Nuclease Assay D-2
Geometric (Exponential) Phase D-4
kinetic analysis of D-4
Linear Phase D-5
Plateau Phase D-5
SYBR Green Chemistry D-3
PCR step
fill reservoirs, checking after centrifuging 4-18
fill reservoirs, trimming after sealing 4-21
general practices 4-6
placing in bucket in centrifuge 4-17
TaqMan Low Density Array, centrifuging 4-16
TaqMan Low Density Array, loading 4-14
TaqMan Low Density Array, placing in
bucket 4-16
TaqMan Low Density Array, sealing 4-19
persisted data
analysis sessions 1-28
plate documents
1-28
studies 1-28
pipetting errors 8-6
pipettors, using 8-6
plate adapter, changing 7-12
plate document information
applying to a plate document 3-28
plate documents 3-9
about 2-12
adding to the plate queue 4-35, 4-40
applying detector tasks 3-16
applying sample names 3-28
assigning standard quantities 3-17
closing 2-18
configuring document information 3-28
copying detectors 3-13
copying markers 3-15
creating 2-13, 3-9
creating from a template 3-26, 4-36
exporting 2-14
importing setup data 2-16, A-2
methods, See methods
opening 2-19
programming methods 3-19
removing from the plate queue 4-40
running batches 4-39
running individually 3-29
saving 2-17
saving as a single plate file 4-24
saving as a template file 3-24
saving to the database 4-24
setting Sample Volume 3-22
setting the passive reference 3-18
plate grid
about 2-29
selecting wells 2-24
viewing well information 2-23
zooming 2-26
Plate Handler 1-8, 7-36
adjustment knob 7-36
aligning 7-41 to 7-48
cleaning the finger pads 7-52
gripper 7-36
plate stack positions 7-36