Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Index-1
Index
Symbols
– 6-31
↑ 6-33
Numerics
7900HT instrument, See instrument
9600 emulation mode 3-21
A
ABI PRISM 7700 Sequence Detection System,
emulating 3-21
ABI PRISM Sealer, see Sealer
absolute quantification
about 6-6 to 6-7
analyzing data 6-9 to 6-13
assay development guidelines B-6
procedure checklist 6-9
selecting and preparing standards B-6
troubleshooting 8-12
adding
bar code to a plate document 2-27, 3-28
custom dyes to the pure dye set 7-27
detector tasks to a plate document 3-16
detectors to a plate document 3-13
markers to a plate document 3-15
plate documents to the plate queue 4-35, 4-40
sample names to a plate document 3-28
adjusting
analysis options for absolute quantification 6-11
display settings 3-24
method step parameters 3-21
plate-sensor switch 7-37 to 7-40
adjustment knob 7-36
air bubbles 8-6
aligning
fixed-position bar code reader 7-49 to 7-51
Plate Handler 7-41 to 7-48
allele calls
about 5-15
calling 5-14
scrutinizing 5-16
allelic discrimination
about 5-6 to 5-8
analyzing run data 5-10 to 5-18
assay development guidelines B-5
maximizing throughput 3-4
procedure checklist 5-10
thermal cycling on the 7900HT instrument 3-20
troubleshooting 8-13
Allelic Discrimination Plot
about 5-13
calling alleles 5-14
datapoint cluster variations 5-8
exporting as a graphic file A-16
exporting data as a text file
A-16
genotypic segregation 5-8
outliers 5-8
scrutinizing allele calls 5-16
amplification
beyond reproducible limits in multi-reporter
experiments 6-19
amplification curve
about D-4
Geometric (Exponential) Phase D-4
Linear Phase D-5
Plateau Phase D-5
Amplification Plot
exporting as a graphic file A-16
exporting data as a text file A-16
Analysis 6-22
analysis sessions, about 1-28
analyzing
absolute quantification data 6-9 to 6-13
allelic discrimination data 5-10 to 5-18
background data 7-19
dissociation curve data 6-40 to 6-44
pure dye data 7-25
relative quantification data 6-22 to 6-35
applying
detector tasks 3-16
detectors to a plate document 3-13
markers to a plate document 3-15
sample names 3-28
architecture
database LAN setup 1-23
database WAN setup 1-24
Archiver 1-26
archiving files 7-54
arrows, sample bars 6-31
assay development guidelines
absolute quantification B-2 to B-4, B-6
allelic discrimination B-2 to B-5

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English