Bruker BioSpin Solid State NMR - Page 131

HETCOR with DUMBO, PMLG or w-PMLG, Using Shapes
User Manual Version 002 BRUKER BIOSPIN 131 (327)
sp0 Power level channel 2 for contact pulse.
spnam0 ramp.100 or similar Shape for contact pulse channel f2.
sp1 set to pl13 To match cnst20.
spnam1 lgs-2 To match ppg calculation of in0, in_f1.
p3 2.5 – 3 µsec 90º pulse channel 2 at pl12.
p15 50 -500μs Contact pulse width.
pcpd2 ≈ 2*p3 SPINAL64 /TPPM decoupling pulse.
cpdprg2 SPINAL64/TPPM15 Decoupling sequence.
F1
1
H indirect
l0 0 Start value 0, incremented during expt.
l3 2- 4 Multiples of FSLG-periods, increment per row.
in_f1 =in0 as calculated Set according to value calculated by ased.
F2
13
C acquisition
d1 2s Recycle delay.
sw 310ppm Sweep width direct dimension.
aq 16-20 msec
masr 10-15 kHz 15 kHz ok at 100 kHz RF-field.
Table 9.2. Processing Parameters for pmlg-HETCOR (as for FSLG on tyrosine-HCl)
Parameter Value Comment
F2 direct dim
13
C
si 2-4 k
wdw QSINE SSB 2, 3 or 5.
ph_mod pk
F1 indirect
1
H
si 256-1048
mc2 STATES-TPPI
wdw QSINE
ssb 3, 5
ph_mod pk
Table 9.1. Acquisition Parameters for pmlg-HETCOR (on tyrosine-HCl)