Bruker BioSpin Solid State NMR User Manual

260 (327) BRUKER BIOSPIN User Manual Version 002
Double-CP
Table 20.1. Recommended Parameters for the DCP Setup
Parameter Value Comments
Pulse program doubcp AVIII, Topspin 2.1 only, else use doubcp, doubcp.av
nuc1
13
C Nucleus on f1 channel
o1p 100 ppm
13
C offset
nuc2
1
H Nucleus on f2 channel
o2p 2-4 ppm
1
H offset, optimize
nuc3
15
N Nucleus on f3 channel
o3p ≈35 glycine
≈120 histidine
≈65 -130 protein
15
N offset depending on sample
sp1 Power level for f1 channel, NC contact pulse
pl3 Power level for
15
N channel HN contact
pl5 Power level for
15
N channel, NC contact pulse
pl12 Power level decoupling f2 channel and excitation
pl13 power level during second contact, cw dec.
cnst24 offset for cw decoupling during p16
p3 Excitation pulse f2 channel
pcpd2 Decoupler pulse length f2 channel (
1
H) TPPM
p15 3-5 msec Contact pulse – first contact
p16 5-12 msec Contact pulse – second contact f1 – f3 channel
d1 5-10s histidine
4s α-glycine
Recycle delay
spnam0 Ramp for 1
st
CP step; e.g. ramp: 80 – 100%
sp0 Power level for Ramp HN contact pulse 1H
spnam1 ramp45-55, tcn5500, or
square.100
ramp, tangential contact pulse tcn5500
on C, or square
spnam2 square.100 or ramp45-
55, tcn5500
square on N, or ramp/tangential pulse
cpdprg2 SPINAL64 SPINAL64 decoupling
ns 2, 4, or 16 Number of scans

Bruker BioSpin Solid State NMR Specifications

General

Brand	Bruker BioSpin
Model	Solid State NMR
Category	Measuring Instruments
Language	English