PISEMA
User Manual Version 002 BRUKER BIOSPIN 197 (327)
Table 15.1. Acquisition Parameters
Parameter Value Comments
PULPROG Pisema, pisema-
clean
Pulse programs.
NUC1 15N
SW Reasonable SW in F2.
O1P 90 – 160 ppm For 15N labeled acetylated glycine.
NUC2 1H
O2P to be optimized For 15N labeled acetylated glycine.
PL1 For 15N contact.
PL11 Or 15N evolution.
PL2 For 1H contact and excitation.
PL12 For 1H heteronuclear decoupling during t2.
PL13 For 1H Evolution under FLSG condition.
P3 1H excitation pulse.
P15 15N-1H Contact pulse.
P6 1H LG 294 degree pulse.
D3 1.4 µs For frequency & phase setting D*X only.
cnst20 1H spin nutation frequency achieved with PL13.
cnst21 0 Offset from o2 in Hz.
cnst22 + LG frequency in Hz calculated.
cnst23 - LG frequency in Hz calculated.
L3 1 – 3 Loop counter for appropriate t1 increment.
F2 acquisition 15N ************** (left column).
AQ_MOD qsim
TD 512 No of points.
DW Dwell time in t2.
F1 indirect 1H ************** (right column).
TD 64 Number of points.
IN_F1 L3*2*p5*SF or
L3*2.5*p5*SF
Scaling factor for PISEMA 0.82 = sin(54.7 deg.) calcu-
lated by pulse program.
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