134 (327) BRUKER BIOSPIN User Manual Version 002
Modifications of FSLG HETCOR
dumbohet 9.2.4
This is the windowed version of the previous experiment, analogous to wpmlghet.
Run xau dumbo, select d (for dumbo), 1 (for 1 cycle), 0 (for added angle). The
calculated shape, dumbo_1+0 will be entered as spnam1, p10 will be set to 32
µsec. The projection of this experiment can be compared to the result of a direct
proton detected CRAMPS experiment, using dumbod2. At higher fields, p10 may
have to be set to 24 rather than 32 µsec for better resolution. The same pulse
length p10, the same shape, the same window (p9) and the same power level
should be used in both experiments.
The resolution along the proton dimension is comparable for all these experi-
ments. The FSLG experiment is the most forgiving, requiring just the knowledge
of the RF power level for decoupling at a certain RF field. Setting cnst20 to this
RF-field (+ 5 or 10%) is all that needs to be set, if the
13
C observe parameters are
well adjusted.
F2
13
C acquisition
d1 2s Recycle delay.
sw 310 ppm Sweep width direct dimension.
aq 16-20 msec
masr 12000-13000
Table 9.5. Acquisition Parameters for DUMBO-HETCOR (on tyrosine-HCl)
Parameter Value Comments
pulprog dumbohet Windowed dumbo shape.
nuc1 13C
o1p 100 ppm
nuc2 1H
cnst24 1000-3000 Place carrier within proton spectrum for evolution.
pl1 Power level channel 1 for contact pulse.
pl12 Power level channel 2 TPPM/SPINAL decoupling.
pl13 Power level channel 2 DUMBO decoupling.
sp0 Power level channel 2 for contact pulse.
spnam0 ramp.100 or similar Shape for contact pulse channel f2.
sp1 set to pl13 100 kHz for default duration 32 µs.
spnam1 dumbo_1+0 Both include one DUMBO cycle.
p3 2.5 – 3 µsec 90º pulse channel 2 at pl12.
Table 9.4. Acquisition Parameters for e-DUMBO-HETCOR (on tyrosine-HCl)
To Next Page
Loading...