132 (327) BRUKER BIOSPIN User Manual Version 002
Modifications of FSLG HETCOR
w-pmlghet 9.2.2
If one wants to compare a solids proton spectrum, acquired via
13
C detection (us-
ing HETCOR) and a direct detect proton spectrum (using w-pmlg), it is useful to
do this using exactly the same parameters (power levels and timings) in both ex
-
periments. If w-pmlg is used and optimized for the direct detect experiment, the
same parameters will work with w-pmlghet, provided that both experiments are
done with the same probe.
Table 9.3. Acquisition Parameters for wpmlg-HETCOR (on tyrosine-HCl)
Parameter Value Comments
pulprog wpmlghet Phase ramp allows detection window.
nuc1 13C
o1p 100 ppm
nuc2 1H
cnst20 usually >=100 kHz As prepared with wpmlgd proton detect exp.
cnst24 1000-3000 Place carrier within proton spectrum for evolution.
pl1 Power level channel 1 for contact pulse.
pl12 Power level channel 2 TPPM/SPINAL decoupling.
pl13 Power level channel 2 w-PMLG decoupling.
sp0 Power level channel 2 for contact pulse.
spnam0 ramp.100 or similar Shape for contact pulse channel f2.
sp1 set to pl13 To match cnst20.
spnam1 m5m or m5p Both include one FSLG cycle.
p3 2.5 – 3 µsec 90º pulse channel 2 at pl12.
p15 50 -500 µsec Contact pulse width.
pcpd2 ≈ 2*p3 SPINAL64 /TPPM decoupling pulse.
cpdprg2 SPINAL64/TPPM15 Decoupling sequence.
F1
1
H indirect
l0 0 Start value 0, incremented during expt.
l3 2- 4 Multiples of FSLG-periods, increment per row.
in_f1 =in0 as calculated Set according to value calculated by ased.
F2
13
C acquisition
d1 2s Recycle delay.
sw 310ppm sweep width direct dimension.
aq 16-20 msec
To Next Page
Loading...