Applied Biosystems 7900HT - Page 382

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Index-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
single-plex TaqMan reagents 4-11
TaqMan primers and probes 4-10
trimming after sealing 4-21
TaqMan reagents, description 4-10
TaqMan RNase P Instrument Verification Plates
about 7-30
analyzing 7-34
kits C-5
preparing a plate document 7-33
running 7-30, 7-32
Targets, mRNA, number per well 4-10
tasks, See detector tasks
templates
about 2-12
creating a single plate document 3-26
creating multiple plate documents 4-36
saving as 3-24
thermal cycler block, See sample block module
thermal cycling protocol, See methods
threshold
about D-6
configuring for automatic analysis 6-11
improper setting 8-5
threshold cycle
calculation D-6
relationship to PCR product D-7
Time readout (from the Real Time tab) 4-27
Time Remaining readout (from the Real Time
tab) 4-27
T
m
, See melting temperature
troubleshooting 8-2 to 8-20
7900HT instrument 8-14
background runs 8-9
chemistry problems 8-5 to 8-8
computer 8-14
end-point runs 8-13
fixed-position bar code reader 8-17, 8-20
pure dye runs 8-11
real-time runs 8-12
SDS software 8-14
Zymark Twister Microplate Handler 8-17, 8-20
turning ON the Applied Biosystems 7900HT Fast Real-
Time PCR System 2-4
U
upgrading
operating system software 7-55
SDS software 7-55
V
viewing, well information 2-23
views
hiding 2-21
maximizing/minimizing 2-22
resizing 2-21, 2-22
showing 2-21
W
warranty statement E-1
well inspector 2-31
Y
Y Inter readout (from the Standard Curve Plot) 6-13
Z
zooming
allelic discrimination plot 5-14
plate grid wells 2-26
Zymark Twister Microplate Handler
See Plate Handler
Zymark Twister Software
about 1-14
aligning the Plate Handler 7-41 to 7-48
closing 7-48
launching