Applied Biosystems 7900HT - Page 381

DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Index-7
sample block locking bar 7-8
sample block locking bolt 7-8
sample block module
about 1-5
cleaning sample block module wells 7-15
contamination 8-7
replacing 7-6
sample names
adding to a plate document 3-28
importing into a plate document A-2
Sample readout (from the Real Time tab) 4-27
sample volume setting 3-22
saturation, signal 7-27
saving
plate documents 2-17, 4-24
template files 3-24
scrutinizing allele calls 5-16
SDS 7900HT Document (*.sds) files
See plate documents
SDS 7900HT Template Document (*.sdt) files
See templates
SDS Enterprise Database
about 1-22
command line tools 1-26
RQ Manager Software 1-25
saving plate documents 4-24
saving results 5-19, 6-53
saving templates 3-25
SDS Database 1-26
SDS User Account Manager 1-27
SNP Manager Software 1-25
study-centric design 1-27
SDS software
about 1-14
disconnecting 4-30
installing 7-55
launching 2-7
learning to use the software 2-10
re-connecting 4-30
upgrading 7-55
Sealer
installation 4-14
overview 4-14
Sealer, see microfluidic card sealer
Sealing
guidelines 4-19
TaqMan Low Density Array 4-19
trimming fill reservoirs after sealing 4-21
selecting
wells from the plate grid 2-24
Service Name A-22
setup table file
about A-4
example files A-4
exporting
A-17
importing into a plate document A-2
structure A-4
signal saturation 7-27
Slope readout (from the Standard Curve Plot) 6-13
SNP Manager Software 1-25
spectral calibration, See pure dye runs
Stage readout (from the Real Time tab) 4-27
Standard Curve Method 6-4
Standard Curve Plot
about 6-13
exporting as a graphic file A-16
exporting data as a text file A-16
standards
quantifying for absolute quantification B-6
selecting for absolute quantification B-6
selecting for relative quantification B-6
starting
plate queue 4-43
run from the SDS software 4-25
State readout (from the Real Time tab) 4-27
Status readout (from the Real Time tab) 4-27
step
adding to a method 3-21
Step readout (from the Real Time tab) 4-27
stopping
plate queue 4-44
run from the SDS software 4-28
studies 1-28
study-centric design 1-27
SYBR Green 1 Dye
about D-3
System performance, verifying
RNase P card, preparing 7-32
T
table pane
about 2-29
exporting A-16
TaqMan fluorogenic probe
about D-2
designing B-2 to B-3
TaqMan Low Density Array
centrifuging 4-16
components 4-10
handling 4-14
how it works 4-10
loading 4-14
loading guidelines 4-14
number of targets per well 4-10
overview 4-10
placing in bucket 4-16
quality control 4-12
removing from packaging 4-14
sealing 4-19