82 (327) BRUKER BIOSPIN User Manual Version 002
Basic Setup Procedures
Note that the spectral window (swp) is set in ppm which makes the acquisition
time dependant on the B
0
-field at a given td of 2k. This is intended and accounts
for the linewidths dependence on the B
0
-field. The glycine lines show a broaden-
ing proportional to B
0
due to chemical shift dispersion. To make S/N values more
comparable, this accounts for shorter T
2
at higher field.
Table 4.1. Summary of Acquisition Parameters for Glycine S/N Test
Parameter Value Comments
PULPROG cp cp.av for AV1 and 2
NUC1 13C Nucleus on f1 channel
O1P 100 ppm
13
C offset
NUC2 1H Nucleus on f2 channel.
D1 4 s Recycle delay.
NS 4 Number of scans.
SWP 300 ppm Spectral width for Glycine.
TD 2048 Number of acquired complex points.
CPDPRG2 SPINAL64 Decoupling scheme f2 channel (
1
H).
SPNAM0 ramp.100 or ramp 70100.100 For ramped CP.
P15 2 ms Contact pulse (f1 and f2 channel).
PL1 Set for 4-4.5 µsec P90.
SP0 (or pl2 AV1+2) Set for 4-4.5 µsec P90 – 2 dB (optimize).
PL12 High power level f2 channel (
1
H) excitation
and decoupling.
P3 90
o 1
H pulse at PL12 (f2 channel).
PCPD2 or
P31 (AV1+2)
SPINAL64 decoupling pulse.
O2P 2.5 - 3 ppm
1
H offset - optimize in 400 - 500 Hz steps for
maximum signal of aliphatic peak.
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