Siemens Rapidlab 1200 - Recovery and Precision in Human Whole Blood

Rapidlab 1200 Operator’s Guide: System Specifications E-33
02087462 Rev.V
Recovery and Precision in Human Whole Blood
To prepare samples for analysis, blood was collected in heparinized vacuum tubes
and tonometered at 37
°C to each of 6 levels to prepare samples for pH, Na
+
, K
+
,
Cl
-
, and Ca
++
, 5 levels to prepare samples for glucose and lactate analysis, and 9
levels to prepare samples for pCO
2
and pO
2
analysis. Multiple runs were
performed using these samples on a minimum of 3 Rapidlab
1260 systems
The following tables summarize the results of the Rapidlab 1260 system human
whole blood recovery precision testing.
pO
2
1 213 154.1 0.86 1.34
pO
2
2 213 103.3 1.19 1.27
pO
2
3 213 67.3 1.80 2.14
* WRSD = within-run standard deviation
† Total SD = total standard deviation
Table E-34 Rapidlab 1260 Recovery and Precision Testing—pH
Level Mode N WRSD
*
Observed Expected Recovery WRCV
1 Capillary 24 0.003 7.056 7.048 100.11 0.04
2 Capillary 24 0.001 7.228 7.231 99.96 0.02
3 Capillary 24 0.005 7.317 7.311 100.09 0.07
4 Capillary 25 0.001 7.433 7.423 100.14 0.02
5 Capillary 24 0.002 7.539 7.526 100.17 0.02
6 Capillary 24 0.002 7.650 7.637 100.16 0.03
1 Syringe 46 0.008 7.038 7.048 99.87 0.11
2 Syringe 48 0.003 7.232 7.231 100.02 0.05
3 Syringe 48 0.005 7.310 7.311 99.99 0.07
4 Syringe 46 0.003 7.424 7.423 100.02 0.04
5 Syringe 51 0.002 7.525 7.526 99.98 0.03
6 Syringe 45 0.003 7.633 7.637 99.95 0.03
1Micro-
capillary
12 0.002 7.040 7.048 99.88 0.03
2Micro-
capillary
12 0.002 7.138 7.175 99.48 0.02
3Micro-
capillary
12 0.003 7.343 7.338 100.06 0.04
4Micro-
capillary
12 0.001 7.430 7.420 100.13 0.01
Table E-33 Rapidlab 1260 AutomaticQC Precision Results
Analyte Level N Mean WRSD
*
Total SD
†