E-46 Rapidlab 1200 Operator’s Guide: System Specifications
02087462 Rev.V
Method Comparison with Human Whole Blood Samples
For testing syringe and capillary modes, blood was collected in heparinized vacuum tubes.
It was tonometered at 37°C and/or adjusted chemically. Several levels of tHb were
prepared by separating the red cells from the plasma and recombining appropriately.
Specimens were assayed on Rapidlab
1260 and Rapidlab 865 systems.
Method comparison was quantified by obtaining estimates of proportional (slope), and
constant (intercept) bias, root mean square error (RMSE), and method correlation (R
square for r
2
coefficient of determination) by performing an ordinary least squares
analysis of the Rapidlab 1260 (dependent variable) versus the Rapidlab
865 (independent
variable). Results are summarized in the table below.
4 pH, Glu,
Lac
Syringe
46 0.15 11.3 12.6 89.8 1.3
5 pH, Glu,
Lac
Syringe
47 0.36 15.6 18.0 86.6 2.3
* WRSD = within-run standard deviation
Table E-43 Rapidlab 1260 Human Whole Blood Method Comparison—pH
Mode N Slope Intercept RMSE r
2
Min Max
Capillary 145 1.001 -0.003 0.009 0.998 7.042 7.684
Syringe 284 1.015 -0.108 0.008 0.998 6.991 7.676
Micro-
capillary
72 0.989 0.080 0.006 0.999 7.030 7.651
Micro-
syringe
143 0.950 0.361 0.059 0.911 7.030 7.651
pH Only
Capillary
143 1.001 -0.003 0.007 0.999 7.015 7.639
pH Only
Syringe
273 1.004 -0.035 0.009 0.998 7.004 7.642
pH, Glu,
Lac
Capillary
144 1.001 -0.000 0.007 0.999 7.046 7.657
pH, Glu,
Lac
Syringe
253 1.006 -0.045 0.006 0.999 7.045 7.660
Table E-42 Rapidlab 1260 Recovery and Precision Testing—Lactate
Level Mode N WRSD
*
Observed Expected Recovery WRCV
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