Siemens Rapidlab 1200 - Reference Methods

E-72 Rapidlab 1200 Operator’s Guide: System Specifications
02087462 Rev.V
Reference Methods
The following reference or comparative methods were used to generate data for the
Rapidlab
1200 systems.
Analyte Reference Method
pH Internal ISE method used.
pCO
2
and pO
2
Tonometered whole blood. Refer to CLSI document C46-A.
2
Gases
used are traceable to NIST Certified Reference Material SRM 1701.
Na
+
and K
+
Internal ISE method used.
Cl
-
Coulometric reference method.
Ca
++
Internal ISE method used.
Glucose The hexokinase/glucose-6-phosphate dehydrogenase method
described in CLSI document RS1-A.
4
Lactate Internal method using lactate oxidase assay.
tHb Cyanmethemoglobin Reference Method per Clinical and Laboratory
Standards Institute (CLSI), approved reference procedure
5
using a
spectrophotometer.
FO
2
Hb Tonometry, where whole blood samples are tonometered with 95%
O
2
, and 5% CO
2
.
FHHb Tonometry, where whole blood samples are tonometered with 95%
N
2
, and 5% CO
2
.
FCOHb
Reduced gas Chromatography.
6
FMetHb
Modified Evelyn-Malloy
7
method utilizing a spectrophotometer.