Rapidlab 1200 Operator’s Guide: System Specifications E-25
02087462 Rev.V
Recovery and Precision of Hemoglobin Fractions in Human
Whole Blood
For testing capillary, tHb, and syringe modes, blood is collected in heparinized
vacuum tubes. It is tonometered at 37°C and/or adjusted chemically. Three target
levels of tHb were prepared by separating the red cells from the plasma and
recombining appropriately. For each run, separated by mode, 3 replicates of each
level were run on a minimum of 3 Rapidlab
1245 systems. The mean bias was
determined by taking the difference between the Rapidlab
1245 system mean and
the reference methods mean. The mean bias and the pooled within run standard
deviation were determined for each blood preparation and sampling mode.
5Micro-
capillary
12 1.86 150.3 149.8 100.3 1.2
6Micro-
capillary
12 2.21 186.7 199.5 93.6 1.2
7Micro-
capillary
12 3.70 239.9 249.7 96.1 1.5
8Micro-
capillary
12 9.31 332.2 377.6 88.0 2.8
9Micro-
capillary
12 10.50 556.7 649.3 85.7 1.9
1Micro-
syringe
24 0.33 27.5 28.3 97.0 1.2
2Micro-
syringe
24 0.59 51.7 50.1 103.3 1.1
3Micro-
syringe
24 0.79 91.3 85.9 106.3 0.9
4Micro-
syringe
24 0.74 106.0 107.4 98.7 0.7
5Micro-
syringe
24 1.06 150.6 150.3 100.2 0.7
6Micro-
syringe
24 13.25 190.7 198.2 96.3 6.9
7Micro-
syringe
24 3.57 247.5 250.5 98.8 1.4
8Micro-
syringe
24 6.96 341.4 375.1 91.0 2.0
9Micro-
syringe
24 8.38 583.6 651.3 89.6 1.4
* WRSD = within-run standard deviation
Table E-20 Rapidlab 1245 Recovery and Precision Testing—pO
2
Level Mode n WRSD
*
Observed Expected Recovery WRCV
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