Waters Autospec Premier - Page 17

Table of Contents 3
Setting the maximum retention time ............................................................. 3-6
Setting up scanning functions ....................................................................... 3-7
Magnet Scan function...................................................................................... 3-7
Voltage Scan function.................................................................................... 3-10
B/E (product ion) function ............................................................................. 3-12
B2/E (precursor ion) function ........................................................................ 3-15
Constant Neutral Loss (CNL) function......................................................... 3-18
MIKES Scan function .................................................................................... 3-21
NRMS Scan function ..................................................................................... 3-22
SIR function editors ....................................................................................... 3-24
The voltage SIR function editor .................................................................... 3-25
The SIR Mass dialog box .......................................................................... 3-29
The MRM function editor .............................................................................. 3-30
The MRM Mass dialog box ....................................................................... 3-33
Example of VSIR experiment set up ........................................................... 3-34
Introduction.................................................................................................... 3-34
Creating the VSIR function........................................................................... 3-35
Example of MRM experiment set up .......................................................... 3-39
Creating the MRM function .......................................................................... 3-39
Applying an inter-scan current in FI ionization mode .......................... 3-43
4 Acquiring data ........................................................................................ 4-1
Starting data acquisition ................................................................................ 4-1
Starting an Acquisition from the Tune window............................................. 4-1
Starting multiple-sample data acquisition from the MassLynx window ..... 4-5
Automatic quantification of the Sample List ................................................. 4-9
Stopping data acquisition ............................................................................. 4-11
Pausing data acquisition ............................................................................... 4-12
Monitoring data acquisition ......................................................................... 4-12
Viewing the status of an acquisition............................................................. 4-12
Viewing a chromatogram in real-time.......................................................... 4-13
Viewing a spectrum in real-time................................................................... 4-13