Waters Autospec Premier - Example of MRM Experiment Set up; Creating the MRM Function

Example of MRM experiment set up 3-39
Example of MRM experiment set up
Multiple Reaction Monitoring (MRM) is used for monitoring high energy ion
fragmentation reactions. You can monitor a specified number of
fragmentation reactions by setting the magnet’s field strength to a fixed value
and changing the ESA voltages with the accelerating voltage at close to its
maximum.
To use the MRM function the Hall probe on the instrument must be set up for
MRM operation by a qualified service engineer. Contact your local service
representative for assistance if you need to activate this functionality.
The sample being analyzed is passed through a GC column, with its
constituent components eluting at different times, that is, the GC column has
a separating effect on chemicals of differing structure. Hence, the MRM
acquisition is set up according to these elution (retention) times.
See “The MRM function editor” on page 3-30.
Creating the MRM function
To open the MRM function editor
1. In the MassLynx window, click the MS Method icon.
2. In the MS method editor, click .
Result: The default function no longer appears in the function list.
3. Click .
Alternative: Click Functions > MRM.