Applied Biosystems 3500 - Evaluate the Spatial Calibration Profile

The display updates as the run progresses.
A Spatial QC Check error message is display
ed if:
• The average peak height or individual peak height is below the threshold
• Uniformity or capillary spacing exceeds the threshold
When the run is complete:
1.
Evaluate the spatial calibration prole to ensure that you see:
• One sharp peak for each capillary. Small shoulders are acceptable.
• One marker (+) at the apex of every peak. No o-apex markers.
• An even peak prole (all peaks about the same height).
2.
If the results meet the criteria above, click Accept Results.
If the results do not meet the criteria above, the Accept buon is dimmed. Go to
“Spatial calibration troubleshooting“ on page 271.
IMPORTANT! Do not log o or close the softw
are before clicking Accept Results.
Spatial calibration results are not saved until you click Accept Results.
Evaluate the
spatial c
alibration
profile
Chapter 6 Run c
alibrations and install checks
Run a spatial calibration
6
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
105