Applied Biosystems 3500 User Manual

The Capillary Run Data display (Figure 10) is updated after the run is complete:
•
The spectral calibration status is displayed in the rst row of the run results table.
Passing and failing capillaries in the install check run are shown in green and red
respectively for the CRL (contiguous read length) criteria. Borrowed capillaries
(spectral only) are shown in yellow with an arrow indicating the adjacent
capillary from which results were borrowed. The spectral result for each capillary
is displayed below the run results table.
• The sequencing install standard status is displayed in the third row of the run
results table (CRL Pass/Fail).
• The Quality Value and Condition Number for each capillary is displayed below
the table.
Note: The values shown in this gure are examples only.
Figure 10 Capillary Run Dat
a
The software evaluates the Quality Value and Condition Number for each capillary
(for more information, see “Spectral Quality Values and Condition Numbers“ on
page 113).
Borrowing is automatically enabled. 1 borrowing event is allowed for 8-capillary
instruments. Up to 3 borrowing events are allowed for 24-capillary instruments. For
more information, see “Spectral calibration with Borrowing enabled“ on page 116.
The number of borrowing events can be decreased (see “System preferences“ on
page 37).
Thresholds used by the software for pass/fail are listed in the following table:
Dye set Quality Value minimum
Condition Number
maximum
E 0.95 5.5
Z 0.95 5.5
Pass/fail criteria
f
or the optional
spectral
calibration
Chapter 6 Run c
alibrations and install checks
Run a Sequencing install check
6
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V