Applied Biosystems 3500 User Manual

Symptom
Possible cause Action
AnyDye Set Spectral Calibration fails. Problem with spectral calibration See “Perform a spectral
c
alibration“ on page 111.
AnyDye dye set is not set up correctly. See “Create a new dye set using the
AnyDye template“ on page 168.
Sequencing install standard troubleshooting
Symptom
Possible cause Action
No signal Incorrect preparation of standard Replace samples with fresh samples
pr
epared with fresh Hi‑Di
™
Formamide (see “Hi‑Di
™
Formamide“ on page 22 for storage
conditions).
Bubbles in sample wells Centrifuge samples to remove
bubbles.
Capillaries are not aspirating sample Check that sample volume is at least
10 µL. If sample volume is adequate,
contact Thermo Fisher Scientific.
The capillary tips may be hitting the
bottom of the wells. Autosampler not
correctly aligned.
Contact Thermo Fisher Scientific.
The Sequencing install check fails:
Failed capillaries
• One or more (for 8-capillary).
• Three or more (for 24-capillary).
Accept button is not active, Reject
button is active.
Blocked capillary Run the Fill Array with Polymer
wizard. Install a new capillary array.
Insufficient filling of array. Check for broken capillaries. Run the
Fill Array with Polymer wizard.
Expired sequencing standard or old
reagents.
Check the expiration date and storage
conditions of the sequencing
standard and/or reagents. If
necessary, replace with a fresh lot.
Bubbles in the polymer system. Run the Remove Bubbles wizard.
Appendix A Troubleshoot
Sequencing install standard troubleshooting
A
274
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V