Setup workflow
1. “Prepare the instrument“ on page 41
2. “Prepare sample plates“ on page 52 and “Load the plate in the
instrument“ on page 56
3. “Check system status in the Dashboard“ on page 32
q q
1. “Create or import a plate“ on
page 42
2. “Assign plate contents“ on
page 44
3. “Print the plate layout“ on
page 50
4. “Link the plate“ on page 57
“Quick Start a run“ on page 58
q q
1. “Load plates for run and create the injection list“ on page 59
2. “Review and modify the injection list in Preview Run“ on page 62
3. “Start the run from Preview Run“ on page 64
4. “Check sequence or sample quality and re-inject samples“ on
page 67
Prepare the instrument
1.
In the Dashboard, check consumables status (see “Check consumables status“ on
page 33). Ensure that:
• Consumables are not expired
• Adequate injections remain for consumables
2.
Ensure that buer levels are at the ll lines (see “Check buer ll levels“ on
page 34).
3.
Set the oven temperature, then click Start Pre-heat.
Pre-heat the oven and detection cell while you prepare for a run (detection cell
temperature is set by the software). Pre-heating helps mitigate subtle rst-run
migration rate eects. The pre-heat function automatically turns o after 2 hours.
We recommend that you pre-heat the oven for at least 30 minutes before you
start a run if the instrument is cold.
Temperatures are displayed in red as they warm to the set-points. When
temperatures are at the set-point they are displayed in green. Temperatures may
uctuate slightly when they reach the set-point as they stabilize.
4.
Check the pump assembly for bubbles and run the Remove Bubble wizard if
needed (see “Remove bubbles from the polymer pump“ on page 237).
Chapter 3 Set up and run
Setup workflow
3
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
41
To Next Page
Loading...
Need help?
General
