Applied Biosystems 3500 User Manual

Note: Spatial and spectral calibration reports include the date on which a capillary
array is installed for the rst time on the instrument. Install standard reports use the
most recent install date if a capillary array w
as removed and re-installed on the
instrument.
1.
Click
V
iew Report.
2.
(Optional) In the Report screen, click toolbar options to manipulate the report.
Place the mouse pointer over an item for a description of the item.
3.
To print the report, click
Print.
IMPORTANT! Sav
e a report electronically for record keeping. The software does not
save historical results. Only the most recent results are maintained in the software.
1.
Click V
iew Report.
2.
Click
Print.
3.
In the Printer dialog box, select CutePDF W
riter as the printer.
4.
Specify a name and location for the report.
Run a spectral calibration
A spectral calibration creates a de-convolution matrix that compensates for dye
overlap (reduces raw data from the instrument) in the dye data stored in each sample
le.
IMPORTANT! T
o calibrate a custom dye set using AnyDye, rst create the dye set,
then select the name of the custom dye set from the Dye Set list. The AnyDye selection
in the Dye Set list contains default seings. It does not correspond to custom dye sets
created with the AnyDye dye set template.
Perform a spectral calibration when you:
•
Use a dye set that you have not previously calibrated
• Replace the capillary array for maintenance purposes
• Replace the capillary when it expires (the expiration date is indicated on the
packaging and the RFID tag)
Note: When the instrument reads the information from a newly installed
capillary array, you are required to run a spatial calibration and a spectral
calibration before you can run plates.
• See a decrease in spectral separation (pull-up/pull-down in peaks) in the raw or
analyzed data
Note: For sequencing applications, you can skip this process, and run the Sequencing
install check. If you select Keep Spectral Calibration Data in the Install Check, the
software runs a spectral calibration for the dye set during a sequencing check and
View and print a
calibration report
Save historical
r
eports (.pdf) for
record keeping
Spectral
c
alibration
overview
When to perform a
spectr
al
calibration
Chapter 6 Run c
alibrations and install checks
Run a spectral calibration
6
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
107

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V