Applied Biosystems 3500 - CHAPTER 7 Manage Library Resources; Overview of Libraries

Manage library resources
■
Overview of libraries ................................................ 138
■
General library procedures ........................................... 139
■
Plates library ....................................................... 142
■
Assays library ...................................................... 144
■
File Name Conventions library ........................................ 147
■
Results Group library ................................................ 152
■
Instrument protocol library ........................................... 161
■
Dye sets library ..................................................... 165
■
Size standards library ................................................ 170
■
Basecalling protocols library (primary analysis—sequencing) ............. 173
■
Sizecalling protocols library (primary analysis—fragment) ................ 178
■
QC protocols library (primary analysis—HID) .......................... 185
Overview of libraries
The Library workow contains the following libraries:
• Items that you select when you set up a run:
– Plates—Contains factory-provided plate templates that you can use to create
plates for each run.
– Assays—Contains factory-provided assay templates that you cannot modify.
You can also create new assays.
– Optional Filename Conventions—Contains factory-provided le name
conventions that you cannot modify. You can also create new le name
conventions.
– Optional Results Groups—Contains factory-provided results groups that
you cannot modify. You can also create new results groups.
• Items that you select when you create an assay:
– Instrument protocols
– Primary analysis protocols—Basecalling (sequencing), sizecalling (fragment
analysis), QC (HID analysis)
• Items you select when you create instrument sizecalling and QC protocols:
– Dye sets
– Size standards
7
138
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1