Applied Biosystems 3500 User Manual

Setting
Description
Normalization Factor
Thresholds
The passing range for Normalization Factor (default range is 0.3 to 3.0).
IMPORTANT! Incr
easing the factor threshold above 3.0 may cause amplification of
noise.
If the calculated Normalization Factor is outside the Normalization Factor range, the
softwar
e multiplies the peak heights of the sample by the low or high Normalization
Factor threshold setting (for example, if the Normalization Factor range is 0.3 to 3.0
and the calculated Normalization Factor is 5, the software applies a Normalization
Factor of 3.0).
Normalization Factor Average peak height of the subset of peaks in the GS600 LIZ
™
v2 size standard used for
normalization divided by the Normalization Target. Samples are flagged with
in
r
esults if Normalization Factor is within threshold range, or with
if it is out of
thr
eshold range.
Dye sets library
A dy
e set denes the following for an instrument protocol:
• Dye color(s)
• Order of the dye peaks in the standard
• Spectral analysis parameters
When you create an instrument protocol, you add a dye set to the protocol. If you add
this item from the library, a copy of the item is added to the assay, and can be modied
independently from the original item stored in the library. For information on how
changes are tracked if auditing is enabled, see “Audit action“ on page 205.
Dye set overview
Chapter 7 Manage libr
ary resources
Dye sets library
7
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Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V