Setting
Description
Locked When SAE is enabled, allows the entry to be unlocked and modified only by the user
who created it, the administrator, or another user with unlock permissions. Useful
when your system includes the SAE module (described in Chapter 8, “Use Security,
Audit, and E-Sig functions (SAE Module)“.
Description Optional text entry.
Oven temperature (°C) Temperature setting for main oven throughout run.
Run voltage (kVolts) Final sample electrophoresis separation run voltage.
Prerun voltage (kVolts) Pre run voltage setting before sample injection.
Injection voltage (kVolts) Injection voltage setting for sample injection.
Run time (sec) Length of time data is collected after voltage is ramped up to the run voltage and the
run starts.
PreRun time (sec) Prerun voltage time.
Injection time (sec) Sample injection time.
Data delay (sec) Time from the start of separation to the start of sample data collection.
Advanced options - Do not change unless advised otherwise by Life Technologies support personnel
Voltage tolerance (kVolts) Maximum allowed voltage variation.
Voltage # of Steps (nk) Number of voltage ramp steps to reach Run Voltage.
Voltage step interval (sec) Dwell time at each voltage ramp step.
First read out time (ms) The interval of time for a data point to be produced. First ReadOut time should be equal
to Second ReadOut time.
Second read out time (ms) The interval of time for a data point to be produced. Second ReadOut time should be
equal to First ReadOut time.
Fragment and HID protocols only: Normalization par
ameters - Leave at default settings (for information on how
these parameters are used, see “Review normalized data“ on page 96).
Normalization Target The expected average RFU for the subset of peaks in the GS600 LIZ
™
v2 size standard
used for normalization.
The default value for each run module has been experimentally determined based on
the average peak height of selected peaks in the GS600 size standard with a specific
injection time.
IMPORTANT! If y
ou change the injection time in an instrument protocol, adjust the
Normalization Target proportionately. For example, for an instrument protocol with an
injection time of 10 seconds and a Normalization Target of 2000: if you change the
injection time to 15 seconds (50% increase), change the Normalization Target to 3000
(50% increase).
Chapter 7 Manage library resources
Instrument protocol library
7
164
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
To Next Page
Loading...
Need help?
General
