Applied Biosystems 3500 User Manual

Spectral calibration troubleshooting
Symptom
Possible cause Action
No signal Incorrect preparation of sample Replace samples with fresh samples
prepared with fresh Hi‑Di
™
Formamide (see “Hi‑Di
™
Formamide“ on page 22 for storage
conditions).
Bubbles in sample wells Centrifuge samples to remove
bubbles.
Capillaries are not aspirating sample Check that sample volume is at least
10 µL.
If sample volume is adequate, contact
Thermo Fisher Scientific.
The capillary tips may be hitting the
bottom of the wells. Autosampler not
correctly aligned.
Contact Thermo Fisher Scientific.
Peak heights in the Spectral report
are different from the values seen
when viewing the spectral data in the
electropherogram display.
The raw data electropherogram
display in the software does not have
the Run Scale Divisor applied to the
data. The final peak height values
displayed in the Spectral report have
the Run Scale Divisor applied.
No action.
The Spectral peaks in the raw data
view appear to be in the wrong order
or there are extraneous peaks
Septa contamination. Replace the CBC septa.
IMPORTANT! Mak
e sure to replace
the CBC septa as part of monthly
maintenance.
Appendix A Troubleshoot
Spectral calibration troubleshooting
A
272
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V