The software generates an electropherogram (intensity plot) for each dye based on the
migration of DNA fragments ov
er the run and generates primary analysis results:
• For sequencing applications, the electropherogram is adjusted to compensate for
slight mobility dierences due to the dyes, then basecalling is performed and
quality values are assigned.
• For fragment and Human Identication (HID) analysis, the software uses the
internal size standard to assign a fragment size and a sizing quality value to each
peak.
Materials for routine operation
All materials for routine operation are provided when the instrument is installed. For
more information:
• See Appendix D, “Catalog numbers“
• Contact your local Thermo Fisher Scientic representative
Instrument consumables handling, usage limits, and expiration
IMPORTANT! Before handling chemicals, read and understand all applicable Safety
Data Sheets (SDSs) and use appropriate personal protectiv
e equipment (gloves,
gowns, eye protection, etc). To obtain SDSs, see “Documentation and support“ on
page 310.
Containers and pouches are ready-to-use. Labels include a radio frequency
identication (RFID) tag that the instrument uses to track usage and expiration date.
For application-specic reagents, consumables, and run modules, see
Appendix B,
“Run modules and dye sets“.
Buffers
Cat. No.
Description Storage
4393927 Anode Buffer Container (ABC) 1X running
buff
er, 4 containers
2–8°C
The 1X running buffer has been qualified to ship at
ambient conditions. For a description of the
qualification, go to
http://find.thermofisher.com/ce/ambientbuffers/
email?CID=fl-we18868.
4408256 Cathode Buffer Container (CBC) 1X running
buffer, 4 containers
Results
Chapter 1 Instrument and software description
Materials for routine operation
1
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
19
To Next Page
Loading...
Need help?
General
