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Applied Biosystems 3500 User Manual

Applied Biosystems 3500
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The software generates an electropherogram (intensity plot) for each dye based on the
migration of DNA fragments ov
er the run and generates primary analysis results:
For sequencing applications, the electropherogram is adjusted to compensate for
slight mobility dierences due to the dyes, then basecalling is performed and
quality values are assigned.
For fragment and Human Identication (HID) analysis, the software uses the
internal size standard to assign a fragment size and a sizing quality value to each
peak.
Materials for routine operation
All materials for routine operation are provided when the instrument is installed. For
more information:
See Appendix D, “Catalog numbers“
Contact your local Thermo Fisher Scientic representative
Instrument consumables handling, usage limits, and expiration
IMPORTANT! Before handling chemicals, read and understand all applicable Safety
Data Sheets (SDSs) and use appropriate personal protectiv
e equipment (gloves,
gowns, eye protection, etc). To obtain SDSs, see “Documentation and support“ on
page 310.
Containers and pouches are ready-to-use. Labels include a radio frequency
identication (RFID) tag that the instrument uses to track usage and expiration date.
For application-specic reagents, consumables, and run modules, see
Appendix B,
“Run modules and dye sets“.
Buffers
Cat. No.
Description Storage
4393927 Anode Buffer Container (ABC) 1X running
buff
er, 4 containers
2–8°C
The 1X running buffer has been qualified to ship at
ambient conditions. For a description of the
qualification, go to
http://find.thermofisher.com/ce/ambientbuffers/
email?CID=fl-we18868.
4408256 Cathode Buffer Container (CBC) 1X running
buffer, 4 containers
Results
Chapter 1 Instrument and software description
Materials for routine operation
1
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
19

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Applied Biosystems 3500 Specifications

General IconGeneral
Number of capillaries8
Capillary Length50 cm
LaserSolid-state laser
Instrument TypeCapillary electrophoresis system
TechnologyCapillary Electrophoresis (CE)
Weight95 kg
Power Requirements100–240 V

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