Applied Biosystems 3500 User Manual

• QC—One-page bar chart that shows trace score statistics and results for each
selected sample.
• Plate—One-page per plate for all selected samples that shows the w
ell-location
thumbnail raw data traces with color-coded headers that reect Trace Score
quality.
• Trace Score, CRL, and QV20+—One-page bar chart that shows trace score, CRL,
or QV20+ statistics and results for each selected sample.
• CRL Distribution—One-page bar chart that shows CRL statistics and CRL
results distribution for all selected samples.
• Signal Strength—One-page graph that shows the average sequencing dye signal
strength for all selected samples.
Export sequencing results
1.
Filter the table of interest.
2.
Select an export option: Results, Reports, or Traces.
3.
Select the export options and the location for the export le, then click OK.
The le(s) are exported to the specied location with the following naming
conventions:
• Results—export_ReportName.txt
• Reports—ReportName.* (* is the format you selected: .txt, .xls, .pdf,
or .html)
• Traces—FileName.* (* is the format you
selected: .annotation.txt, .phd.1, .scf, .fsta, .qual, or .seq)
Modify sequence data
To edit, modify, or further analyze sequence data, import the sample data les into a
secondary analysis software application such as SeqScape
™
Software 3 (or later),
MicroSEQ
™
ID Analysis Software v3.0 (or later), Variant Reporter
™
Software 2 (or
later), and Sequence Analysis (SeqA) Software 6 (or later).
Report options
Chapter 4 Re
view sequencing results
Export sequencing results
4
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V