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Applied Biosystems 3500 User Manual

Applied Biosystems 3500
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Run
module
type
Run module
name
Configuration 23 hours throughput
[1]
Performance
Cap.
length
(cm)
Pol.
type
Run
time
(min)
3500
(8-cap.)
3500xL
(24-
cap.)
Range
[2]
Sizing Precision
[3]
50bp-
400bp
401bp-
600bp
601bp-
1200bp
HID HID36_POP4
HID36_POP4xl
36 POP-4
£35 ³312 ³936 £60
to ³400
<0.15 NA
[4]
NA
[4]
SNaPshot
SNaPshot50_PO
P7
SNaPshot50_PO
P7xl
50 POP-7
£30 ³376 ³1104 £40
to ³120
<0.50 NA
[4]
NA
[4]
[1]
T
hroughput (Samples / Day): The total number of samples run in 23 hours (0.5 hour for user interaction and 0.5 hour for warm-up time).
[2]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 or
GS1200 LIZ size standard sample sized with a third order fit) is ³1. The table shows the resolution range in ³90% of samples.
[3]
Sizing Precision: Standard deviation of sizes for one allele in the DS-33 install standard sized with the GS600 LIZ size standard across multiple
capillaries in the same run. For one injection to pass, 100% of the alleles in that injection must meet the intra-run sizing precision specifications.
The table shows the sizing precision of 100% of alleles in ³90% of samples.
[4]
Not applicable because of the size of the fragments collected in the run.
Dye sets
Table 7 Sequence analysis dye sets
Dye Set Application Name
E (v1.1 BigDye
T
erminator) Rapid DNA sequencing
Z (v3.1 BigDye
Terminator) DNA sequencing
Table 8 F
ragment analysis dye sets
Application
E5 SNaPshot
kit
G5 DNA sizing for 5-dye chemistry
J6 DNA sizing for 6-dye chemistry
F DNA sizing for 4-dye chemistry
D DNA sizing for 4-dye chemistry
AnyDye DNA sizing
Sequencing
anal
ysis dye sets
Fragment analysis
dye sets for all
applications
Appendix B Run modul
es and dye sets
Dye sets
B
284
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

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Applied Biosystems 3500 Specifications

General IconGeneral
Number of capillaries8
Capillary Length50 cm
LaserSolid-state laser
Instrument TypeCapillary electrophoresis system
TechnologyCapillary Electrophoresis (CE)
Weight95 kg
Power Requirements100–240 V

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