Applied Biosystems 3500 User Manual

Setting
Description
Do you wish to assign
multiple instrument
protocols to this assay?
When you select Yes, allows you to select or create additional instrument protocols for
the assay. The software creates one injection for each instrument protocol specified in
an assay.
Instrument Protocol Instrument protocol for data collection.
F
or information, see “Instrument protocol settings“ on page 163.
Basecalling Protocol
(sequencing)
Protocol for basecalling, trimming, and quality determination.
For information, see “Basecalling protocol—Analysis settings“ on page 176.
Sizecalling Protocol
(fragment analysis)
Protocol for primary analysis (peak detection and sizing) and quality determination.
For information, see “Sizecalling protocol—Analysis settings“ on page 181 .
QC Protocol (HID) Protocol for primary analysis (peak detection and sizing) and quality determination.
For information, see “QC protocols library (primary analysis—HID)“ on page 185.
File Name Conventions library
A File Name Conv
ention (FNC) species the naming convention for sample data les.
It is an optional component in a plate.
If you do not specify a le name convention, data les are named in this format:
<sample name>_<well>
The le extension is determined by the application you run:
• Sequencing—.ab1 (you can also set Preferences to export additional le formats.
See “Set preferences (optional)“ on page 36.)
• Fragment analysis—.fsa
• HID—.hid
Note: The le location specied in a le name convention is used only if a results
group is not specied for a well.
File name
convention
overview
Chapter 7 Manage libr
ary resources
File Name Conventions library
7
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
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Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V