Applied Biosystems 3500 User Manual

Section 6.2 Run an install check
Run a Sequencing install check
If an install check is not performed when your instrument is installed, you must
perform an install check before you can run plates.
We recommend that you run an install check monthly to verify that the instrument
meets specications.
The sequencing install check has an option to include and save the spectral
calibration. If you select this option and you accept the sequencing install standard
results, you do not need to run a separate spectral calibration (described in “Run a
Sequencing install check“ on page 121) for E or Z dye set.
Note: The run times listed below do not include the time for the oven to preheat to
60°C.
• General sequencing (BDTv3.1 on POP-7
™
polymer): ~1 hour
• MicroSeq ID: 2 hours
• BDTv1.1POP6: ~2.5 hours
• BDTv1.1: ~2.5 hours
Before you begin
If you have not already done so, perform a spectral calibration (see “Perform a
spectral calibration“ on page 111).
Prepare the instrument
1.
In the Dashboard, check consumables status (see “Check consumables status“ on
page 33). Ensure that:
• Consumables are not expired
• Adequate injections remain for consumables
2.
Ensure that buer levels are at the ll lines (see “Check buer ll levels“ on
page 34).
3.
Set the oven temperature, then click Start Pre-heat.
Pre-heat the oven and detection cell while you prepare for a run (detection cell
temperature is set by the software). Pre-heating helps mitigate subtle rst-run
migration rate eects. The pre-heat function automatically turns o after 2 hours.
We recommend that you pre-heat the oven for at least 30 minutes before you
start a run if the instrument is cold.
Temperatures are displayed in red as they warm to the set-points. When
temperatures are at the set-point they are displayed in green. Temperatures may
uctuate slightly when they reach the set-point as they stabilize.
4.
Check the pump assembly for bubbles and run the Remove Bubble wizard if
needed (see “Remove bubbles from the polymer pump“ on page 237).
When to perform a
sequencing ins
tall
check
Estimated run
time
Prepare for the
sequencing install
check
Chapter 6 Run calibrations and install checks
Run a Sequencing install check
6
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Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V