Applied Biosystems 3500 - Page 122

Prepare the sequencing install check standard
Prepare the BigDye
™
Terminator v1.1 or v3.1 Sequencing Standard as described in the
product insert. See Appendix D, “Catalog numbers“ for catalog numbers.
Note: For the BigDye
™
Direct DNA PCR Amplication/Clean-up/Cycle Sequencing
kit, use the v3.1 Sequencing Standard.
Prepare the standard plate
IMPORTANT! Do not use warped or damaged plates.
1.
Load the standards in any injection position in the plate. The example below
shows injection position 1, but y
ou can specify the starting well for an injection
position.
IMPORTANT! You do not create a plate in the software for the install check.
8-capillary
96-well plate
A1 through H1
24-capillary
96-w
ell plate
A1 through H1, A2 through H2, and A3 through H3
24-capillary
384-well plate
Note: 384-well
plates are not
supported on
8‑capillary
instruments.
Columns 1, 3, and 5 in rows A, C, E, G, I, K, M, O
2.
Briey centrifuge the plate that contains the standards.
Chapter 6 Run c
alibrations and install checks
Run a Sequencing install check
6
122
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1