Applied Biosystems 3500 - Results Group for One Allelic Ladder Per Run Folder; Prepare Sample Plates

For a 24-capillary instrument, create a results group that species an injection folder
,
then select this results group for all injections on the plate.
For an 8-capillary instrument, create one results group for each set of three injections
on the plate (each results group species a results group name folder). For more
information, see “Results Group example 2: store re-injections in separate folders“ on
page 158.
1.
Pipet samples into the plate according to the plate layout (see “Print the plate
layout“ on page 50).
2.
Briey centrifuge the plate.
3.
Verify that each sample is positioned correctly in the boom of its well.
IMPORTANT! If the contents of any w
ell contain bubbles or are not located at the
boom of the well, briey centrifuge the plate, remove the plate from the
centrifuge, and verify that each sample is positioned correctly in the boom of its
well.
4.
Store the plate on ice and protected from light until you prepare the plate
assembly and load the plate in the instrument.
Results group for
one allelic ladder
per run folder
Prepare sample
plates
Chapter 3 Set up and run
Prepare and load sample plates
3
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1