Applied Biosystems 3500 User Manual

allows you to save the spectral calibration data. For information, see “Run an install
check“ on page 121.
Not
e: The run times listed below do not include the time for the oven to preheat to
60°C.
Application Standard Polymer type Run time (min)
Sequencing Sequencing
standard
POP-6
™
polymer £135
Sequencing Sequencing
standard
POP-7
™
polymer £40
Fragment analysis Matrix standard Any polymer £75
Before you begin
If you have not already done so, perform a spatial calibration (see page 104).
Prepare the instrument
1.
In the Dashboard, check consumables status (see “Check consumables status“ on
page 33). Ensure that:
• Consumables are not expired
• Adequate injections remain for consumables
2.
Ensure that buer levels are at the ll lines (see “Check buer ll levels“ on
page 34).
3.
Set the oven temperature, then click Start Pre-heat.
Pre-heat the oven and detection cell while you prepare for a run (detection cell
temperature is set by the software). Pre-heating helps mitigate subtle rst-run
migration rate eects. The pre-heat function automatically turns o after 2 hours.
We recommend that you pre-heat the oven for at least 30 minutes before you
start a run if the instrument is cold.
Temperatures are displayed in red as they warm to the set-points. When
temperatures are at the set-point they are displayed in green. Temperatures may
uctuate slightly when they reach the set-point as they stabilize.
4.
Check the pump assembly for bubbles and run the Remove Bubble wizard if
needed (see “Remove bubbles from the polymer pump“ on page 237).
Prepare the spectral calibration standard
Prepare the matrix or sequencing standard appropriate for your application as
described in the product insert. See Appendix D, “Catalog numbers“ for catalog
numbers.
If peaks are oscale for G5, F, and E5 dye sets, dilute the matrix standard and repeat
the calibration.
Estimated run
time
Prepare for
spectr
al
calibration
Chapter 6 Run calibrations and install checks
Run a spectral calibration
6
108
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V