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Applied Biosystems 3500 User Manual

Applied Biosystems 3500
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1.
Do either of the following:
Create a plate on another 3500 Series Data Collection Software 3.1 computer,
then export (see “Import and export a plate“ on page 79).
Create a plate import le (see “Create a plate import le“ on page 78.
IMPORTANT! If you copy/paste sample or plate information into the Assign
Plate Contents screen or into a plate import le, copy from a plain text editor
such as Notepad. Do not copy from a word processing program such as
Microsoft
Word
, which may include invisible, non-ASCII characters. Non-
ASCII characters in plate or sample information may cause a run to stop or may
prevent a run from starting.
2.
Access the Assign Plate Contents screen: Click the W
orkow tab in the
Dashboard, then select Assign Plate Contents in the navigation pane.
3.
Click
Import, then select the plate import le.
4.
Click Assign Plate Contents.
As
sign plate contents
You assign the following information to the wells in a plate before you can run the
plate:
Sample names and sample types (required)—Identies the well positions of each
sample for data collection and processing.
Assay (required)—Species the parameters that control data collection and
primary analysis (basecalling or sizing). All named wells on a plate must have an
assigned assay. For more information on assays, see “Assays library“ on page 144.
Filename convention (optional)—Species le naming. For more information, see
“File name convention overview“ on page 147.
Results group (optional)—Species sample data le storage. For more
information on assays, see “Results Group overview“ on page 152.
Import a plate
Chapter 3 Set up and run
Assign plate contents
3
44
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Table of Contents

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Applied Biosystems 3500 Specifications

General IconGeneral
Number of capillaries8
Capillary Length50 cm
LaserSolid-state laser
Instrument TypeCapillary electrophoresis system
TechnologyCapillary Electrophoresis (CE)
Weight95 kg
Power Requirements100–240 V

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