Applied Biosystems 3500 - Basecalling Protocols Library (Primary Analysis-Sequencing); Basecalling Protocol Overview; Create a New Basecalling Protocol

Basecalling protocols library (primary analysis—sequencing)
A basecalling protocol is the required primary analysis protocol for sequencing
applications.
A basecalling protocol denes the seings used by the sequencing basecallers to
assign base calls to each detected peak and assign a quality value:
• Analysis seings
• Ranges for the sequencing quality ags displayed in View Results
When you create a sequencing assay, you add a basecalling protocol to the assay. If
you add this item from the library, a copy of the item is added to the assay, and can be
modied independently from the original item stored in the library. For information
on how changes are tracked if auditing is enabled, see “Audit action“ on page 205.
If factory-provided basecalling protocols do not suit your needs, you can create new
basecalling protocols:
1.
Access the Basecalling Protocols library.
2.
Click Create.
3.
In the Analysis Seings tab of the Create New Basecalling Protocol dialog box
(Figure 23), specify seings “Basecalling protocol—Analysis seings“ on
page 176.
Basecalling
pr
otocol overview
Create a new
basecalling
protocol
Chapter 7 Manage library resources
Basecalling protocols library (primary analysis—sequencing)
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