Applied Biosystems 3500 - Page 174

4.
Click QV Seings. In the QV Seings tab of the Create New Basecalling Protocol
dialog box (Figure 24), specify seings (see “Basecalling protocol—QV
seings“ on page 178).
QV seings are quality value ranges used in the following screens:
• Monitor Run screen—The state of the QV ag:
– If all three values are in the pass range, the QV ag in Monitor Run is
set to (green).
–
If any values are in the suspect range, the QV ag in Monitor Run is set
to
(yellow).
–
If any values are in the fail range, the QV ag in Monitor Run is set to
(red).
• V
iew Sequencing Results4Metric Analysis Results table—The
pass/check/fail status for Trace Score Quality, CRL Quality, and QV20+
Quality results.
Chapter 7 Manage libr
ary resources
Basecalling protocols library (primary analysis—sequencing)
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1