4.
Click QV Seings. In the QV Seings tab of the Create New Basecalling Protocol
dialog box (Figure 24), specify seings (see “Basecalling protocol—QV
seings“ on page 178).
QV seings are quality value ranges used in the following screens:
• Monitor Run screen—The state of the QV ag:
– If all three values are in the pass range, the QV ag in Monitor Run is
set to (green).
–
If any values are in the suspect range, the QV ag in Monitor Run is set
to
(yellow).
–
If any values are in the fail range, the QV ag in Monitor Run is set to
(red).
• V
iew Sequencing Results4Metric Analysis Results table—The
pass/check/fail status for Trace Score Quality, CRL Quality, and QV20+
Quality results.
Chapter 7 Manage libr
ary resources
Basecalling protocols library (primary analysis—sequencing)
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1