Applied Biosystems 3500 User Manual

Contents
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CHAPTER 1 Instrument and software description ................... 14
Instrument and software description ............................................. 14
Overview ................................................................. 14
Precautions for use ........................................................ 15
Instrument interior components ............................................. 15
Instrument parts and functions .............................................. 16
Instrument front panel indicators ............................................ 17
Instrument and computer requirements .......................................... 17
Windows
™
software requirements ........................................... 17
Antivirus software requirements ............................................. 17
Other software ............................................................ 17
Instrument firmware ....................................................... 18
Theory of operation ............................................................ 18
Preparing samples ......................................................... 18
Preparing the instrument ................................................... 18
During a run .............................................................. 18
Results ................................................................... 19
Materials for routine operation .................................................. 19
Instrument consumables handling, usage limits, and expiration ..................... 19
Buffers ................................................................... 19
Polymer .................................................................. 20
Conditioning reagent ....................................................... 21
Capillary arrays .......................................................... 21
Hi‑Di
™
Formamide ......................................................... 22
Important notice regarding use of consumables that exceed supported limits ..... 22
Overview of the 3500 Series Data Collection Software 3.1 ........................... 23
About the software ........................................................ 23
Dashboard ............................................................... 23
Workflow ................................................................ 24
Maintenance workflow ..................................................... 25
Library workflow .......................................................... 25
SAE menu ................................................................ 26
Tools menu ............................................................... 26
Manage menu ............................................................. 26
Preferences menu ......................................................... 27
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
3

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V