Applied Biosystems 3500 User Manual

Help menu ................................................................ 27
Use the software without an instrument ...................................... 27
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CHAPTER 2 Start the system .......................................... 28
Workflow ..................................................................... 28
Start the computer and instrument ............................................... 29
Start the software .............................................................. 30
Step one: Start the Server Monitor ........................................... 30
Step two: Start the 3500 Series Data Collection Software 3.1 .................... 31
Step three: Log in .......................................................... 31
Check system status in the Dashboard ............................................ 32
Check calendar reminders .................................................. 32
Check consumables status ................................................. 33
How the polymer sample and injection counters calculate usage ................. 34
Check for leaks and spills ................................................... 34
Check buffer fill levels ...................................................... 34
Replenish consumables .................................................... 35
Ensure proper installation of CBC septa ...................................... 35
Set preferences (optional) ....................................................... 36
Overview ................................................................. 36
System preferences ........................................................ 37
User preferences .......................................................... 37
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CHAPTER 3 Set up and run ............................................ 40
Setup workflow ................................................................ 41
Prepare the instrument ......................................................... 41
Create or import a plate ........................................................ 42
About plate templates ...................................................... 42
Create a plate from a template .............................................. 42
Import a plate ............................................................. 44
Assign plate contents ........................................................... 44
Access the Assign Plate Contents screen ..................................... 45
Name samples and assign sample types in the plate view ....................... 47
Assign assay, file name convention, and results group in the plate view ........... 49
How file location in file name conventions and results groups work .............. 50
Print the plate layout ....................................................... 50
Prepare and load sample plates .................................................. 50
Capillary-to-plate mapping ................................................. 51
Allelic ladder run requirements ............................................. 51
Results group for one allelic ladder per run folder ............................ 52
Prepare sample plates ..................................................... 52
Prepare the plate assembly ................................................. 53
Contents
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V