Applied Biosystems 3500 User Manual

Load the plate in the instrument ............................................. 56
Link the plate ............................................................. 57
Quick Start a run ............................................................... 58
Load plates for run and create the injection list .................................... 59
Link a plate (if a plate is not linked) .......................................... 61
Link a plate from the Recent Plates or Recent Runs tab ......................... 62
Review and modify the injection list in Preview Run ................................ 62
Start the run from Preview Run .................................................. 64
Monitor the run ................................................................ 65
Check sequence or sample quality and re-inject samples ........................... 67
Check sequence or sample quality ........................................... 67
Re‑inject samples from the Monitor Run screen ............................... 69
If you select a protocol other than the original ................................. 70
How re-injections are displayed in the plate view .............................. 70
Re-inject HID allelic ladder samples ......................................... 71
Review completed injections in Review Results .................................... 72
Pause, resume, or stop a run .................................................... 72
Pause and resume ......................................................... 72
Abort or terminate ......................................................... 72
More features in Assign Plate Contents ........................................... 73
Name samples in the plate view ............................................. 73
Customize the plate view ................................................... 74
View the capillary-to-plate map ............................................. 75
Use the table view ......................................................... 75
Sort by one or multiple columns ............................................. 76
Customize a table .......................................................... 76
Add assays, file name conventions, and results groups to a plate ................ 77
Create a plate import template .............................................. 77
Create a plate import file ................................................... 78
Edit a plate ................................................................ 79
Import and export a plate ................................................... 79
Create a plate template ..................................................... 79
Specify the default plate type for the Open Plate dialog box ..................... 80
Save electronic version of reports ............................................ 80
More features in Monitor Run .................................................... 80
Review the Instrument Run views ............................................ 80
Array view ................................................................ 81
Sample view .............................................................. 81
EPT view .................................................................. 81
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CHAPTER 4 Review sequencing results .............................. 82
Workflow ..................................................................... 82
Access the View Sequencing Results screen ....................................... 82
Review results for the currently running sequencing plate .......................... 83
Contents
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
5

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V