Applied Biosystems 3500 - APPENDIX G Normalization; Overview of the Normalization Feature

Normalization
Overview of the normalization feature
For fragment analysis applications, the 3500 Series Data Collection Software 3.1
includes an optional normalization feature for use with the GeneScan
™
600 LIZ
™
Size
Standard v2.0 (GS600 LIZ v2). This feature aenuates signal variations associated with
instrument, capillary array, sample salt load, and injection variability between
capillaries and instruments. Normalization can be applied during primary analysis of
the data.
To use the normalization feature, prepare each sample with the GS600 LIZ v2 size
standard, then specify the appropriate normalization size standard for le primary
analysis. The GS600 LIZ
™
v2 reagent can function as an internal standard for
signal-height normalization as well as a size standard for peak sizing.
G
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
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