Applied Biosystems 3500 User Manual

4.
Invert the ABC, then tilt it slightly to move most of the buer to the larger side of
the container. The smaller side of the container should contain <1 mL of the
buer.
5.
V
erify that the buer is at the ll line.
6.
Peel o the seal at the top of the ABC.
7.
W
ith the RFID label toward instrument, place the ABC into the anode-end of the
instrument, below the pump. Position the anode in the large chamber of the
ABC, then push the ABC up and back to install.
IMPORTANT! The RFID label must be facing the instrument (away from you) to
ensure that the RFID information is read accurately by the instrument.
8.
Close the instrument door to re-initialize.
9.
In the Dashboard, click Refresh, then check the Quick V
iew section for updated
status.
1.
Check the expiration date on the label to ensure it is not expired and will not
expire during use.
2.
Allow refrigerated CBC to equilibrate to ambient temperature.
3.
Wipe away condensation on the CBC exterior with a lint-free tissue.
Condensation can cause arcing and termination of the run.
4.
Check that the seal is intact. Do not use if the buer level is too low or the seal
has been compromised. A ll tolerance of ±0.5 mm is acceptable.
1
1
Fill line
Install the cathode
buff
er container
(CBC)
Chapter 9 Maint
ain the Instrument
Install buffers
9
230
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V