Symptom
Possible cause Action
Electrophoresis current is unstable. Bubbles in the polymer system. Run the Remove Bubbles wizard.
Electrophoresis failure. Buffer below fill line. Ensure that the buffer is filled up to
the fill line. “Check buffer fill
levels“ on page 34 .
There is not enough fluid in larger
chamber of ABC, or the anode buffer
has spilled into smaller overflow
chamber.
Pipette the buffer from the smaller
overflow chamber to the larger
chamber. Ensure that the buffer is
filled to within ±1 mm of the fill line.
When installing new ABC, tilt the
container to move buffer to the larger
side of the container as described
“Install the anode buffer container
(ABC)“ on page 229.
Extra peaks in the electropherogram. Data off scale. Dilute the sample and re-inject the
sample.
Possible contaminant in sample. Re-amplify the DNA.
Sample re-naturation. Heat-denature the sample in good
quality formamide and immediately
place on ice.
Review Results troubleshooting
Symptom
Possible Cause Action
Zoom errors in electropherogram
graphical displays (Monitor Run,
Review Results, Spectral
Calibration, and Install Check):
• The zoom feature does not
re‑baseline the sample data
view,
or
• The X axis of the sample plot
does not stay at the bottom of
the screen. It moves up toward
the region the user has zoomed
in on, making data difficult to
review
The zoom feature does not
re‑baseline the sample data view.
No action.
Samples are not imported when you
select multiple folders for import
At least one file is not in the correct
format for import, therefore no files
are imported.
Select individual folders or files for
import instead of multiple folders.
Appendix A Troubleshoot
Review Results troubleshooting
A
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
267
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