Applied Biosystems 3500 User Manual

Symptom
Possible Cause Action
Plate Owner truncated in Annotation
tab>Run Configuration
Special characters were included
when entering plate information.
Use only alpha-numeric characters
for plate information. Special
characters in plate information fields
may not be correctly displayed in
some software screens.
Sample files are not displayed when
imported
You imported (.hid) files and you did
not click HID Samples.
Click HID Samples.
Peaks are not labeled when you
access the screen
Labels are not automatically applied. See “Label peaks“ on page 98.
x and y scaling plot settings are not
applied when you click Apply
Scaling settings are applied only
when you click Zoom.
Click Zoom.
The sizing quality result reported in
the 3500 Series Data Collection
Software 3.1 differs from the sizing
quality result for reported in the
GeneMapper
™
ID-X Software
You imported (.fsa) files instead of
(.hid) files.
The 3500 Series Data Collection
Software 3.1 does not consider the
presence of broad peaks when
determining sizing quality for
fragment analysis data, therefore the
sizing quality result reported in the
3500 Series Data Collection Software
3.1 will differ from the sizing quality
result reported in the GeneMapper
™
ID-X Software, which considers broad
peaks in sizing quality.
No action.
Sizing Overlay report displays data for
all capillaries including those that fail
sizing
Plots displayed in Sizing Overlay Plot
are based on the samples selected.
Select only the capillaries that pass
sizing to include in the report.
Dye/Sample Peak column values in
an exported sizing table are split in to
two separate columns, the column
name containing the Sample Peak
values is incorrectly named, and the
column headers after Dye/Sample
are shifted one column to the right.
You changed the order of columns
before exporting.
Note: The exported data is accurate,
the column headers are shifted to the
right.
Edit the exported file: Cut/paste
column headers one column to the
right, enter correct headers for the
Dye and Sample Peak columns.
Appendix A Troubleshoot
Review Results troubleshooting
A
268
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V