Applied Biosystems 3500 - When to Use the Normalization Feature

When to use the normalization feature
The 3500 Series Data Collection Software 3.1 provides three normalization size-
standard denition les that you can specify for primary analysis of samples prepared
with the GS600 LIZ v2 size standard and the G5 and J6 dye sets:
• Fragment (POP-6
™
and POP-7
™
polymer):
– GS600LIZ+Normalization
– GS600(60-600)LIZ+Normalization—For applications that have primer peaks
that obscure the 20 and 40-mer peaks of the GS600 LIZ v2 size standard.
• Fragment (POP-4
™
polymer):
– GS600(80-400)LIZ+Normalization
• HID (POP-4
™
polymer):
– GS600(80-400)LIZ+Normalization
Appendix G Normalization
When to use the normalization feature
G
300
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1