Applied Biosystems 3500 - Page 112

5.
For Chemistry Standard and Dye Set, select:
Note: For the BigDye
™
Direct DNA PCR Amplication/ Clean-up/Cycle
Sequencing kit, use dye set Z.
Chemistry standard Dye set Application
BigDye
™
Terminator v1.1 sequencing
standard
E Sequencing
BigDye
™
Terminator v1.1 matrix standard E Sequencing
BigDye
™
Terminator v3.1 sequencing
standard
Z Sequencing
BigDye
™
Terminator v3.1 matrix standard Z Sequencing
DS-32 Matrix Standard Kit (Dye Set F) F Fragment analysis
DS-02 Matrix Standard Kit (Dye Set E5) E5 Fragment analysis
DS-33 Matrix Standard Kit (Dye Set G5) G5 Fragment analysis
DS-36 Matrix Standard Kit (Dye set J6, 6-dye) J6 Fragment analysis
Custom AnyDye Fragment analysis
IMPORTANT! The E-signature function creates a record when a spectral
calibration is performed, but does not record the dye set calibrated. To include
the dye set calibrated in the E-signature record, enter the dye set in the E-Sig
Comments eld.
6.
(Optional) Select Allow Borrowing. Selecting this option instructs the softw
are to
automatically replace information from failed capillaries with information from
an adjacent passing capillary with the highest Quality value. For more
information, see “What you see during a spectral calibration“ on page 115.
7.
Click Start Run. The following occurs:
• If you used the default seing "Perform run 2 and 3 if run 1 fails", the
instrument sets up three injections (see “What you see during a spectral
calibration“ on page 115 for information on the number of injections
performed).
• The Capillary Run Data display updates after each injection is complete.
• The status bar updates during Run 1.
IMPORTANT! The status bar does not update during Run 2 or Run 3.
• Passing and failing capillaries are shown in green and red respectively.
Borrow
ed capillaries are shown in yellow with an arrow indicating the
adjacent capillary from which results were borrowed.
To display the result for each capillary (spectral data, Quality Value, and
Condition Number) below the run results table, click a capillary in the table.
Chapter 6 Run c
alibrations and install checks
Run a spectral calibration
6
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3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1