Flag/ Symbols Description
Offscale
At least one data point in the analysis range has saturated
the CCD camera.
Note: In the Monitor Run screen, an offscale sample is
flagged with .
Spectral Pull-Up
(fragment analysis
only)
At least one peak contains a pull-up peak.
A
pull-up peak
is identified when the peak height of the minor
peak is £X% of and within ±Y data point of the major peak,
where X and Y are values you specify.
Broad Peak (HID
analysis only)
At least one peak exceeds the Broad Peak threshold.
Broad peaks affect Sizing Quality. See Chapter 7, “Manage
library resources“.
Note: The value displayed when you place the mouse pointer
over a Broad Peak flag is an internal value and does not reflect
the peak width.
Normalization
Limit
• —Sample was collected with a normalization size
standard, sample Normalization Factor is within range.
—Sample was collected with a normalization size
standard, sample Normalization Factor is not within
range.
No Data—Normalization is enabled, but Sizing Quality is
.
NO—Sample was not collected with a normalization size
standard.
N/A—Sample was not collected on a 3500 instrument.
For more information, see “Review normalized data“ on
page 96.
Note: If the Sizing Quality is , normalization is not applied,
even if the Normalization Factor is within the normalization
range.
SQ: Sizing Quality
The Sizing Quality is in the Fail or Suspect range. Place
the mouse pointer over a flag to display the Sizing Quality
value for the sample.
3.
Click a ag in the samples table, or select samples in the samples table to display
the associated data in the plot view and sizing table view
.
4.
(Optional) Modify the sample view:
• Right-click the Size Standard eld to view the size standard for a sample.
• Click Minimize and Restore to collapse and expand the samples table.
Chapter 5 Re
view fragment/HID analysis results
Review sample quality
5
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
95
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