Applied Biosystems 3500 - Page 239

2.
Aach the syringe to the forward-facing Luer ing at the top of the pump
block. Hold the ing with one hand while threading the syringe onto the ing
with the other hand.
3.
Open the Luer ing by grasping the body of the ing and turning it to loosen.
4.
Grasp the aached syringe and turn counterclockwise approximately one-half
turn.
5.
Slowly depress the plunger
.
IMPORTANT! DO NOT USE EXCESSIVE FORCE when y
ou push the syringe
plunger as this may damage the trap seals. Take approximately 30 seconds to
ush 5 mL of either distilled or deionized water through the trap.
Note: Because the water trap volume is approximately 325 μL, a relatively small
volume of water is adequate for complete ushing. However, a larger volume
improves ushing as long as force and ow rate are kept within the limits given
above.
6.
Remove the syringe from the Luer ing. Hold the ing with one hand while
turning the syringe counterclockwise with the other hand.
7.
Close the Luer ing by lightly turning clockwise until the ing seals against
the block.
Chapter 9 Maint
ain the Instrument
Maintain the pump
9
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
239